Transglutaminase are a family of enzymes that
catalyze the covalent bond formation between open amine groups. They are widely
used in food industries and their demand rises daily. Though they are available in mammalian tissues, fish and
plants, the
complex separation and purification process led to the search of Microbial
Transglutaminase.
Finding a new microbial source of
transglutaminase and the medium composition for MTGase production were the
purpose of this work. Six Different types of Actinomycetes like strains were
isolated from soil sample and two of them named PG03 and PG06 were selected
based on their ability to produce 23 mg/ml and 21 mg/ml MTGase enzyme
respectively.
Strain PG03 was chosen for further studies and
it was found to be a Streptomyces species. Standard enzyme production media
composition is modified and tested to facilitate the optimized MTGase activity.
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