Transglutaminase (E.C. 2.3.2.13) are a family
of enzymes that catalyze the covalent bond formation between open amine groups.
They are widely used in food industries and their demand rises daily. Though
they are available in mammalian tissues, fish and plants, the complex
separation and purification process led to the search of Microbial
Transglutaminase (MTGase).
Finding a new microbial source of
transglutaminase and the medium composition for MTGase production were the
purpose of this work. Six Different types of Actinomycetes like strains were
isolated from soil sample and two of them named PG03 and PG06 were selected
based on their ability to produce 23 mg/ml and 21 mg/ml MTGase enzyme
respectively. Strain PG03 was chosen for further
studies and it was found to be a Streptomyces species. Standard enzyme
production media composition is modified and tested to facilitate the optimized
MTGase activity.
Strategies like finest nitrogen and carbon
source selection, revealing the key ingredients of media by full factorial
design and their optimal concentration Box-Behnken design were adopted. At the
95% confidence level, second order polynomial model was applied to fit the
research outcome.
No comments:
Post a Comment