Cyanide pollution
of aquatic environment has become a great concern in Nigeria because of the
increase in cassava cultivation. In Nigeria, cassava processing milling plants
are usually situated around streams or rivers such that the waste from each stages
of processing easily find their way into these water bodies as effluents and
waste waters.
Extracellular
rhodanese of Klebsiella edwardsii isolated from Atutulala stream, Modakeke,
where cassava is being processed, was assessed for its bioremediation
potential. Cyanide concentration of the stream was analysed for six months.
Four bacterial
isolates were screened for their ability to degrade free cyanide and the best
strain was further screened for rhodanese producing ability. The enzyme was
purified by 85% ammonium sulphate precipitation and diethyl
aminoethyl-cellulose ion-exchange chromatography.
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