Genetic Diversity And Antioxidant Potential Of Zingiber Montanum

Good quality genomic DNA was extracted from the rhizomes of ten accessions of Cassumunar ginger (Table 1) following the modified CTAB method of Ashraf et al., 2014[12] and PCR amplification reaction was performed in a total volume of 25μl containing 10X PCR buffer, 2mM MgCl2, 0.4mM dNTPs (0.025mM each dNTP), 0.2µM decamer primer (Operon), 0.08U of Taq DNA (New England Biolab) and 25 ng of genomic DNA.

The PCR cycle consisted of an initial denaturation at 94°C for 2 min followed by 45 cycles, each consisting of denaturation at 94°C for 1 min, annealing at 36°C for 1 min and extension at 72°C for 2 min. It was followed by a final extension at 72°C for 10 min. Each RAPD PCR reaction was repeated and observed three times for similar banding pattern.

The PCR products were separated alongside a molecular weight marker (100 bp ladder, Himedia) by 1.5% agarose gel electrophoresis in 1X TBE buffer and gels were photographed in a Gel-documentation system (Vilber Lourmat). The amplified DNA fragments were scored as present (1) or absent (0) and used for statistical analyses. Genetic similarities were calculated by Jaccard’s Similarity Coefficient and a dendrogram was constructed by the UPGMA (Unweighted Pair Group Method with Arithmetic mean) method using MEGA6.

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